Journal: Cell reports
Article Title: Macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure.
doi: 10.1016/j.celrep.2024.114395
Figure Lengend Snippet: Figure 2. scRNA-seq reveals EC subpopulations in the adrenal gland (A) UMAP plot showing 4 major endothelial subpopulations in the adrenal gland: capsular arteriole (orange); medullary ECs (purple); sinusoidal ECs (green); lymphatic ECs (pink). (B) Expression-level scaled heatmap of the top 20 marker genes of 4 major endothelial subpopulations. Scale: dark blue, low expression; milky, moderate expression; red, high expression. (C) Violin plots of normalized scRNA expression profiles of known EC subtype markers in each endothelial subpopulation. (D) UMAP plots of 4 major endothelial subpopulations, color-coded for the expression of the indicated markers. Scale: light blue, low expression; red, high expression. (E–G) Representative images of adrenal gland sections, stained for (E) PLVAP, red) (enriched in sinusoidal vessels) together with AQP1 (green) (enriched in capsular arteriole ECs); pan-EC marker (CD31, red) combined with (F) CDH13 (green) (enriched in medullary vessels), and (G) LYVE1 (green) (enriched in lymphatic vessels). Arrows indicate AQP1+ arterioles in the capsule of the adrenal gland (E), CDH13+ vessels in medulla in (F), and LYVE1+ lymphatic vessels in the cor- ticomedullary boundary in (G). Scale bars, 50 mm. Magnifications of the white boxes are shown at right. Scale bars, 20 mm.
Article Snippet: For total endothelial cell and PLVAP+ endothelial cell percentage analysis, after digestion of adrenal gland, single cell suspensions were incubated in dark for 30 min with antimouse CD31 PE antibody (BD Biosciences 553373, 1:400), anti-mouse PLVAP (Proteintech FITC-65214, 1:400) and anti-mouse CD45 BUV395 antibody (BD Biosciences 563791, 1:400) or anti-mouse CD45 PerCP antibody (BD Biosciences 557235, 1:400).
Techniques: Expressing, Marker, Staining